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Control cultures were treated with dimethyl sulfoxide (DMSO).
Parallel cultures were treated with -JQ1 or -JQ1 in DM.
Cultures were treated with 50 nM DH for 3 days before use.
(a) NSC cultures were treated with cort or vehicle for 75 h.
Cultures were treated with either BMP8 or NGF.Controls received neither factor.
Asynchronous cultures were treated with +/− 5 μg ml−1 bleomycin (1 h 26 °C), before being fixed in methanol.
(c) C2C12 cultures were treated with 100 nM siBrd3, siBrd4 or the negative control siRNA (siCtrl) in GM.
After 24 hours, cultures were treated with siRNAs (100 nM) complexed with Lipofectamine RNAiMAX (Life Technologies) according to manufacturer's protocols.
At 2 days in vitro, cultures were treated with a single dose of 20 ng/ml FGF2.
Sixteen hours later the cell cultures were treated with various concentrations of compounds 7f and 7g.
After 24 h incubation, the cultures were treated with the formulations.
More suggestions(15)
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cultures were imaged with
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