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Mk cultures were seeded at 70,000 cells/mL and cultured for up to 21 days at 37°C in a fully-humidified, 5% CO2 environment.
ESC cultures were seeded at clonal density and cultured for 5 days in ES medium (+/-2 μg/mL DOX), or directly fixed when cultured on feeder cells.
Briefly, overnight bacterial cultures were seeded into fresh tryptic soy broth (TSB).
Cells used for experimental cultures were seeded on Poly-L-lysine coated coverslips, no older than passage six.
Human MSC cultures were seeded in Labtek II four-well glass slides (Nalge Nunc International) at 1×104 cells per well.
Triplicate cultures were seeded at 0.5% ring stage parasites in parasite culture media in the presence or absence of 2 mM allopurinol.
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To obtain such a differentiation state, second passage cultures were seed at 20000 cells/cm2, reached confluence between day 5 and 8 depending on the keratinocyte preparation, and then further incubated for 3 days post-confluence.
The five samples submitted to culturing were seeded on sheep blood agar plates and incubated at 37 °C for 24 48 h.
Exponential phase bacterial pre-cultures were seeded in 12-well plates containing sterile glass coverslips and incubated in C medium at pH 7.5 or 6.4 without agitation, to attain OD600 values of 0.3 and 0.6.
Cells from exponentially growing culture were seeded in appropriate numbers in 60 × 15 mm Petri dishes with 10 ml of appropriate culture medium.
Pellets destined to liquid culture were seeded on BBL Mycobacteria Growth Indicator Tubes (MGIT) supplemented with BACTEC MGIT growth supplement and BBL MGIT PANTA (Becton, Dickinson and Company).
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