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Before each experiment, stock cultures were propagated through two consecutive 24-h growth cycles in NB at 30 °C and then cultivated to the exponential phase (5 h).
EVT cultures were propagated from first trimester placental explants essentially as described [16].
Cultures were propagated by dilution with fresh medium with the corresponding concentrations of drugs.
Before using the pathogens as indicator organisms for testing antibacterial activity of Lp9, the cultures were propagated in BHI broth and incubated at 37°C for overnight.
The remaining NS/PC cultures were propagated either as floating neurospheres or as adherent monolayers with mainteinance of growth factor dependence and multipotentiality at late passage.
Cultures were propagated on a common host (Zea mays L). that was grown in sterilized sand (0.45 to 1 mm dia). in a growth chamber for 4.5 months until sporulation.
Similar(28)
Co-cultures were propagated in RPMI 1640 with 10% bovine serum and 10% human interleukin-2 (Gibco-BRL).
Drug-resistant variants grown in cell culture were propagated at low infectious doses, so that each cell was infected with no more than one virus.
Subsequently, 200 µL of the culture was propagated in 5 mL glucose minimal medium supplemented with lysine.
Becky Matthews – a parent in Kent who has campaigned against the "Kent-test" – said that the competitive culture was propagated throughout Kent.
For the precultures, yeast from a stock culture was propagated on YPD plates at 30°C for two days.
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