Sentence examples for cultures were isolated on from inspiring English sources
Yeast cultures were isolated on the Glucose Yeast Peptone agar medium (HiMedia Laboratories, India).
The cultures were isolated on standard malt yeast extract agar (MYA; per liter demi water: 20 g malt extract, 2 g yeast extract, 20 g agar) [ 22] and stored in -80C in a mixture of pepton-glycerol (30% glycerol, 0.7% neopepton).
Colonies forming a clearing zone in the opaque top layer were selected, and pure cultures were isolated by repeatedly inoculating single colonies on nutrient agar.
For B-cell culture, lymphocytes were isolated on a FICOLL gradient from male B6.SJL spleen followed by a 4 days culture on irradiated fibroblasts stably transfected with the CD40L cDNA 3T3-CD40LL) to generate CD40-B cells [ 28].
After the addition of double stranded RNAs targeting SPS1 to the culture medium, total RNAs were isolated on days 1, 3 and 5 after treatment and subjected to microarray analysis using Affymetrix microchips (GEO accession number: GSE 17685).
For primary neuronal cultures, cortexes from mouse pups were isolated on postnatal day 0 or 1. Dissociated cells were electroporated using Amaxa Nucleofector system and maintained in Neurobasal A medium (Invitrogen) supplemented with B27 (Invitrogen) for 7 days.
No other pathogenic bacteria were isolated on culture.
For each culture, detergent-free lipid rafts were isolated on an OptiPrep gradient after ultracentrifugation and lipid raft fractions were identified by raft ELISA (Without antigen, Figure 4A and with antigen, Figure 4B).
Total RNAs from liver-derived stem cell, neonatal liver (NL), and adult liver (AL) were isolated on primary culture by using Trizol method.
No significant pathogenic organisms were isolated on bacteriological culture of the spleen, rumen or abomasum presumably as a consequence of intensive antimicrobial therapy.
Under maintenance culture conditions, SR and lineage-committed cells (erythroid- or myeloid-committed progenitors [ECPs or MCPs]) co-exist in culture and can be isolated on the basis of their surface phenotype.
