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Myoblast cultures were isolated and cultured by standard techniques [40], which, if treated appropriately, are comprised predominantly of proliferating myoblasts and can provide an accurate readout of the regenerative potential of muscle from the H-FRG1TG mice.
Primary myoblast cultures were isolated and cultured using standard techniques.
The RA cell cultures were isolated and cultured as previously published [ 28, 29].
14 Intestinal epithelial cultures were isolated and cultured as described in Sato et al. 15 Colorectal tumour cell lines were maintained, and cell lysates prepared, as described in Emaduddin et al. 16 For de-novo methyltransferase inhibitor treatments, SW48 cells were plated in six-well plates at 2×10 per well.
Mutant cells from γ-radiation-exposed cultures were isolated by fluorescence-activated cell sorting, cultured, and individual clones expanded.
Primary cardiomyocyte cultures were isolated from postnatal day 0 1 mice or rats using the Worthington neonatal cardiomyocyte isolation system (Worthington Biochemical Corp).
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As others have argued, this can only succeed if cultures are isolated enough to maintain and pass on a central core of traditions that can be modified over time.
Apart from the far-reaching benefits provided by the cultures under study, the source from which the cultures are isolated also evokes a great interest.
Genomic DNA from bacterial cultures was isolated with Qiagen DNeasy columns.
RNA from patterned mESC cultures was isolated using TRIzol reagent (Invitrogen) and purified including DNA digestion using the RNEasy Mini Kit (Qiagen) according to the manufacturers' protocols.
Plasmid DNA from overnight cultures was isolated using Pure Yield™ Plasmid Mini Prep System from Promega.
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