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Cultures were inoculated from an overnight malt agar culture.
All cultures were inoculated to an initial OD600nm of 0.05.
For liquid culture replacement, mycelial cultures were inoculated in potato-dextrose liquid medium (PDB, without agar).
Cultures were inoculated (OD600 = 0.1) and grown to late exponential phase (24 h).
Afterward, microalgae cultures were inoculated in wastewater sample in mixotrophic mode under ambient conditions.
Yeast cultures were inoculated into 10 mL YPD and grown overnight.
Planktonic and biofilm cultures were inoculated similarly, but utilizing different media compositions (THB and BFM, respectively).
Overnight cultures were inoculated on the strips (100 µL each) and incubated at 28 °C.
LB cultures were inoculated to OD580 0.05 using an overnight culture and incubated at 30 °C for 24 h.
10% (V inoculum/V media) microalgae cultures were inoculated in reagent bottles containing 1L wastewater samples, respectively.
Cultures were inoculated from stocks into 10 ml media.
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