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Cell lysates was obtained as described by [ 49] with the following modifications; the volume of the cell cultures were increased 2.5 times to get enough material for 10 or more gels.
The results showed that both the percentages and absolute numbers of iNKT cells in cultures were increased by stimulation with α- l-fucosylceramides with C26:0 (4d) > C18 0 (OH) (4b) > C24 0 (4C24 0
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The FW of cell cultures was increased 1.5- and 2.5-fold at 5 h or 24 h after N replenishment.
With respect to upregulation of system xc−, we did find that xCT mRNA expression in astrocytes and mixed cultures was increased in a time-dependent manner following GD.
After 12 hours of growth (OD730 ≈ 1) the irradiance of large-scale cultures was increased to 400 μmol photons s-1 m-2.
With respect to the former, we did find that xCT mRNA expression in mixed cultures was increased in a time-dependent manner following GD.
Mineralization of the cultures was increased by BMPs and decreased by Noggin, as can be observed from the alizarin red S staining, though to a lesser extent (Fig. 4b, c).
The value of spheroid cultures is increasing quickly due to novel microfabricated platforms amenable to high-throughput screening (HTS) and advances in cell culture.
Table 4 showed that nitrate concentration in the ruminal culture was increased by addition of NaNO3 (P < 0.01), but not by addition of NaNO3 + NH4Cl, neither by addition of NH4Cl (P > 0.05).
There was a 12% reduction (down to 88% from that of control) in the PFUs when the concentration of oxethazaine in the culture was increased from 200 µM to 500 µM.
In both units, collaborative culture was increased through the teamwork component of the RTC work.
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