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All cultures were grown in duplicate.
Control cultures were grown in the absence of Se.
New cell cultures were grown as described above.
These cultures were grown at 30 °C and 160 rpm.
Cultures were grown in shake flasks under aerobic conditions.
The cultures were grown at 37°C for 3 h.
Broth cultures were grown overnight with shaking.
Cultures were grown in Leu- Ura- medium.
The cultures were grown until the growth rate remained constant.
For viability assays, cultures were grown to saturation.
Liquid cultures were grown in a shaking incubator (220 rpm).
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