Sentence examples for cultures were expanded from inspiring English sources

Cell cultures were expanded and cultured for at least a month, and fractions were used for analysis of EBP50 expression by western blotting, with GAPDH expression as a protein loading control.

When cultures were expanded for another 4 weeks, all four cell populations displayed similar CD133 and CD15 distribution (Figure 2B).

During a 7 to 35 day analysis period, cultures were expanded as required to keep cells in exponential growth.

Four hybridoma clones were positive, their cultures were expanded, and the antibodies from conditioned media concentrated over protein G columns.

For bulk selection of stably transfected cells, cultures were expanded in the presence of 1 mg/mL G418 and maintained at a concentration of 0.2 mg/mL G418.

Cultures were expanded in flasks and continuously subcultured in the presence of antibiotics and analyses performed as soon as possible following.

The cultures are expanded under serum-free conditions in Neurobasal A medium with addition of the mitogens Epidermal growth factor and Fibroblast growth factor 2 as well as the supplements Glutamax-1 and B27.

Our understanding of development can provide some guidance but since little is known about the specifics of human neural development and the requirement that cultures be expanded in vitro prior to use, it is unclear whether neural progenitors obey the same developmental mechanisms that exist in vivo.

Cultures are expanded to confluency and lifted from the dish substrate by lowering the temperature to 20°C for 2 hours.

Meanwhile, Hovhaness's interest in other eastern cultures was expanding and, in 1940, he decided to destroy a whole host of earlier works with which he was dissatisfied.

HS293 and HS346 were grown on human foreskin fibroblasts, and colonies of cells were subsequently removed from the feeder layer and the culture was expanded at 37°C in a 5% CO2 humidified incubation chamber in serum-free DMEM/F12+ glutamax medium supplemented with B27 (1∶50) heparin (5 µg/ml), antibiotic-antimycotic mixture (1∶100) and EGF + bFGF (20 ng/ml each, Sigma, St . Louis MO, USA).