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The cultures were enriched with f/2 medium as described by Guillard (1975).
Experimental cultures were enriched with CO2 and air mixes of 400 µatm (current) pCO2 and 900 µatm (expected 2100,) and were gradually acclimated to 31°C (1°C increase per week).
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Starting from an inoculum of activated sludge, a DNRA culture was enriched with nitrite as electron acceptor (Fig. 1b).
In the present work, we tested if anchorage-independent cell culture techniques allow the generation of spheroid-cultures and if these cultures were enriched for cells with functional and phenotypic properties characterizing CSC of HNSCC.
Cultures were enriched for G2 cells by inhibiting Cdk1 with RO-3306 (Vassilet et al., 2006 ).
Four different cultures were enriched.
The cultures were enriched by two more rounds of sub-culturing.
As in our previous study, 35 the rate of collagen type-1 synthesis was measured by the use of specific antibodies (enzyme-linked immunosorbent assay) on eight cell cultures, four of which were enriched with 10 μg/mL of the product directly introduced in the culture medium.
Artemia (8 h post-hatch) were enriched with Ori-Culture for 8 h, and then with Ori-Green for 12 h, before being fed to cod larvae.
Rotifers (Brachionus plicatilis) were enriched with Ori-Culture (ORI-GO, Skretting, Vervins, France) (0.25 0.35 g million−1 rotifers) for 4 days, and then with Ori-Green (ORI-GO, Skretting) (0.15 0.25 g million−1 rotifers) for 2 h prior to being fed to cod larvae.
CFs were enriched with the preplating, and cultured on uncoated culture dishes in a 37°C, 5% CO2 incubator.
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