Sentence examples for cultures were determined using from inspiring English sources
The approximate yields of the rhamnolipid compounds in the solvent extracts obtained from the ST5 cultures were determined using the rhamnolipid standard.
The approximate yields of the surfactin compounds in the solvent extracts obtained from the ST34 cultures were determined using the surfactin standard.
IFN-γ measurements in the mouse splenocyte cultures were determined using an EKISA kit (Biosource, Camarillo, CA).
Viability and apoptosis in MoDCs cultures were determined using the Annexin V-PE apoptosis detection kit from Pharmingen (BD Pharmingen) according to the manufacturer's instructions.
Numbers of viable cells in triplicate 100 µl aliquots of cultures were determined using CellTiter-Blue® reagent (CellTiter-Blue® Cell Viability Assay, Promega, UK).
Cell density of Fe(III -grown cultures were determIII -grown epifluoresculturescroscopy were acrideterminede staining [ 79].
The total protein concentration from the collected 1 mL cell pellets obtained from the overall cultures was determined using the bicinchoninic acid (BCA) assay from Pierce (Rockford, USA) and protein expression was further analyzed using 15%% SDS-PAGE.
Cytotoxicity of GCV on cell cultures was determined using Cell Proliferation Kit I (MTT) (Roche, Mississauga, ON) according to the manufacturer's instructions.
