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Positive bacterial cultures were counted as the number of colony-forming units per milliliter, and identification and susceptibility tests were performed according to standard methods.
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However, double positive cells for DiI-Ac-LDL and Lectin in EPC culture were counted as EPCs and used in the present study to analyze the EPC numbers in circulation.
HDFs were collected by trypsinization from cell culture plates and the number of viable cells in the cultures were counted (in triplicates) after Trypan Blue staining using Countess Automated Cell Counter (Invitrogen) and cell counting kit as suggested by the supplier.
Likewise, empiric antimicrobials that were stopped when culture results returned were counted as "nontreated".
In adherent cell cultures, only cells adhering to the culture dish before trypsinization were counted as viable.
For each patient, positive cultures from each site were counted as separate events.
Surviving cells in the culture were counted and expressed as a percent of the number of cells in the 0 hour condition.
Persistent positive cultures from blood or infection sites after 72 hours after receiving daptomycin were counted as microbiological failure [ 13].
The co-culture was then incubated in culture medium without butein for 14 days, after which the numbers of colonies of tumor cells were counted as described above.
Sacrifice flies were counted as at-bats.
These were counted as pursuits.
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