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Virus inoculation of cell cultures was performed using a viral suspension containing homogenized infected mouse brains at a final concentration of 1∶100 (v/v) in culture medium.
Thymidine incorporation in tumorsphere cultures was performed using the trichloroacetic acid (TCA) precipitation method.
Unless noted, plating of pure bacterial cultures was performed using bacteria thawed directly from freezer stocks.
Live imaging of 3D cultures was performed using a Nikon Biostation IM system.
Multidimensional imaging of infected macrophage cultures was performed using a Leica SP5 II Tandem Scanner system (Leica Microsystems).
Knockdown of HPV16 E7 in Ca Ski cell cultures was performed using two different siRNAs, namely si198 and si752.
Similar(42)
Batch cultures were performed using an organic culture broth volume ratio of 1 5 (v/v) for the whole set of the 50 mL cultures.
Blood cultures were performed using an automated system (BACTEC® 9050; Becton-Dickinson, Franklin Lake, NJ, USA).
Quantitative cultures were performed using retrieved samples to determine sterilization effect.
Blood samples were taken on admission for all patients and blood cultures were performed using the BD BACTEC® 9050 blood culture system.
In vitro cultures were performed using purified either LSK (Lin−Sca-1+c-kit+) or CD150+CD48− cells that were described recently (45).
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