Sentence examples for cultures was determined using from inspiring English sources
The total protein concentration from the collected 1 mL cell pellets obtained from the overall cultures was determined using the bicinchoninic acid (BCA) assay from Pierce (Rockford, USA) and protein expression was further analyzed using 15%% SDS-PAGE.
The purity of astrocyte cultures was determined using anti-GFAP antibody.
Cytotoxicity of GCV on cell cultures was determined using Cell Proliferation Kit I (MTT) (Roche, Mississauga, ON) according to the manufacturer's instructions.
The apoptosis and necrosis rate in the incubated cultures was determined using the Annexin-V-Fluos and propidium iodide staining kit (Roche Biosciences) according to the manufacturer's recommendations.
The optimum concentration of HMG for HAC cultures was determined using MTT assay.
Cell density of cultures was determined using cell dry weight (CDW) in g/L.
The approximate yields of the rhamnolipid compounds in the solvent extracts obtained from the ST5 cultures were determined using the rhamnolipid standard.
The approximate yields of the surfactin compounds in the solvent extracts obtained from the ST34 cultures were determined using the surfactin standard.
Viability and apoptosis in MoDCs cultures were determined using the Annexin V-PE apoptosis detection kit from Pharmingen (BD Pharmingen) according to the manufacturer's instructions.
Numbers of viable cells in triplicate 100 µl aliquots of cultures were determined using CellTiter-Blue® reagent (CellTiter-Blue® Cell Viability Assay, Promega, UK).
