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Cells were cultivated for 7 days with an initial cell concentration of approximately 1.0 × 106 cells mL−1 (in mixed cultures, testing all possible associations of two microorganisms, the initial cell concentration was approximately 2.0 × 106 cells mL−1).
Differentiation media consisted of DMEM supplemented with 5% horse serum, 1% penicillin-streptomycin Myoblast cultures testing the presence of recombinant Dlk1 protein were maintained on a bed of 1 mg/mL BD Matrigel (Basement Membrane Matrix; BD Biosciences, San Jose, CA, USA).
Cultures testing positive for influenza A or B viruses were confirmed by using reverse transcription polymerase chain reaction (RT-PCR) analysis with previously reported protocols (16, 17 ).
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Summarizing, anti-adhesive effects of the Rhodococcus biosurfactant depended greatly on bacterial cultures tested and their physiological state.
Results of all stool cultures tested for Campylobacter, Salmonella and Shigella and/or Yersinia spp. were analyzed.
The three cultures tested showed a great variability of ligands that can be produced by different organisms.
Similar results were observed with additional NK cultures tested (data not shown).
Dopamine was detectable by this means in 6 of 10 cultures tested, with a detection limit of ∼40 nM.
Trichodesmium cultures tested under a broader temperature range revealed changes in growth and N2 fixation rates [32].
All blood cultures tested negative.
Within 3 days, the cultures tested positive for acid-fast bacilli.
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