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Catheters inserted and extracted at least 2 days after, in the intensive care unit, are cultured using the quantitative tip cultures technique described by Brun-Buisson.
Conventional blood cultures did not yield satisfactory results, and this led to the development of the classic biphasic blood cultures technique proposed by Ruiz-Castañeda.
Enterotoxins were determined by the Asialoganglioside-GM1 enzyme-linked immunosorbent assay (GM1-ELISA) method using the direct plate cultures technique.
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Embryos were cultured by a modification of the New culture technique (Stern and Ireland, 1981).
In the early 1960s, Brinster developed a culture technique for mammalian eggs that set the standard worldwide.
The culture technique is described as follows.
Selection of potent fungal culture was carried out using enrichment culture technique.
Numerous patents (not listed) demonstrate that Will W. Minuth has invented the presented MINUSHEET® perfusion culture technique.
As a result numerous papers were published dealing with the MINUSHEET® perfusion culture technique.
The isolates were isolated using enrichment culture technique from tannery effluent samples (Sharma and Adholeya 2012).
MINUSHEET® perfusion culture technique was developed to improve the environment of adherent cells and developing tissues.
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