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The nested primers were designed to amplify a lineage within the genus Glomus including G. mosseae, G. caledonium and G. geosporum, all of which had repeatedly been isolated from trap cultures set up with soil from the field.
The majority of CD40-stimulated B cell cultures set up according to this protocol (Fig. 2) has continued to proliferate throughout the observation period.
Similar changes were also seen in non-GFP-labeled parental MDA-MB-435 cells freshly obtained from ATCC and in MDA-MB-435-GFP cell cultures set up at low seeding density (data not shown).
At six weeks, 40 100% of replicate cultures set up with 105 PBMC had given rise to proliferating B cell cultures, and in many donors proliferating cultures could also be obtained from 5 or 2.5×104 PBMC.
However, not all the G2 and MN cultures set up in this study were successful.
All experiments were performed on at least three cultures set up in triplicate.
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Gong Xueping, Shanghai's vice-mayor in charge of culture, set up East Radio and Oriental Television, commercial stations.
Reiss: Specifically how is your culture set up to drive great service?
After all, the culture set up the rules of the game long ago and, in spite of our assertions to the contrary, the economies of power, money and fame depend heavily on appealing to our baser instincts.
We have carried out the neural induction in a 2D culture set up to understand its default state.
IVS was to be performed on 3 occasions, with culture set up on different days.
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