The grafts were cultured in Magenta boxes in a tissue culture room until tissues were collected for RNA extraction.
Cultures were incubated in a culture room at 25 ± 2 °C in dark.
These were incubated in a culture room at 25 ± 2 °C in dark.
All treatments were kept in the tissue culture room for two months.
The plants were grown in a self-regulating culture room with a 12 h/26°C day and a 12 h/20°C night cycle.
The petridishes were incubated in a culture room at 25 ± 2 °C, a light and dark cycle of 16/8 h and an illumination intensity of 34 μmol/m2/s.
Plates were kept vertically in culture room at 29 °C for 12 h of day, 25 °C for 12 h of night and relative humidity at 66%.
All treatments were placed into the tissue culture room (12 h of daylight, 50 μmol m-2 s-1, 26 ± 1°C).
The cultures used were maintained in BG-11 medium in a culture room at 26°C to 30°C with a 16/8-h photoperiod under 3,000 lx from a cool white light.
Orchid seeds were sown on a 1/2 MS (Duchefa Bi ochemia) agar plate containing 0.1%% activated charcoal and placed in a tissue culture room at constant temperature of 24 °C under a 12 h photoperiod.
