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Sampling of the chemostat cultures, probe preparation and hybridization to Affymetrix GeneChip microarrays was performed as described previously [ 12].
For Protocol A, sampling of the chemostat cultures, probe preparation and hybridization to the single-channel Affymetrix GeneChip YG S98 microarrays is described in Piper et al. [ 29].
Sampling of the chemostat cultures, probe preparation and hybridization to single-channel Affymetrix GeneChip YG S98 microarrays was performed as previously described (Piper et al., 2002).
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TB infection was confirmed in bronchoalveolar lavage (BAL) fluid by culture, probe and polymerase chain reaction (PCR).
Despite so much of contemporary popular culture probing h0t-button social issues, there may be no television program that does it with such a fine-tuned hand.
Accordingly, we used time-lapse imaging of rat hippocampal slice cultures to probe for microglial movement associated with SD.
The observation of the presence of mRNA transcripts of CFTR in gene arrays of rat primary cultures (Affymetrix Probe set 1384960_at [ 27]) is consistent with that interpretation.
Cells were used at passages 6 7 in three-dimensional Matrigel matrix cultures to probe the angiogenic potential (tube formation) of roxarsone and AsIII.
Because this in vivo model involves potential indirect effects of blood flow and paracrine-mediated protection from endothelial or other retinal cell types, we used primary RGC cultures to probe the mechanisms of VEGF-A mediated neuroprotection.
Quantification was performed in two independent experiments using different ES cell cultures, different probes and different RNAi treatments.
The cultures were probed for SZP using monoclonal antibody (mAb) S6.79 (a generous gift from Dr. T. Schmid, Rush Medical College, Chicago, IL, USA) with a 1 250 dilution then incubated overnight at 4°C.
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