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The effect of pH was found to be much less than what is observed with suspended cultures of the same biomass.
Transcript and RNA-seq analyses revealed upregulation of key Ca2+-handling, ion channel, and cardiac-specific proteins in hvCOC compared to lower-order 2D and 3D cultures of the same constituent cells.
In 300-mL batch cultures of the same cell line in a TubeSpin® bioreactor 600, 1-L cylindrical glass bottle, and 1-L Erlenmeyer shake flask, the maximum cell densities and recombinant antibody yields were similar.
When comparing expression profiles from replicate tests using the same RNA sample (a test for technical variability), we observed a level of dispersion similar to the pattern obtained with RNA samples from replicate cultures of the same cell line (a test for physiological variability).
However, degradation of quinalphos was slower in the cultures of the same bacterium grown with the amendment of glucose as reflected by the recovery of 24% initially added quinalphos at the end of 2-day incubation.
Immunohistochemical characterization and microscopic analysis of 3-D aggregates of the human intestinal epithelial cell line Int-407 revealed that the 3-D cells more accurately modeled human in vivo differentiated tissues than did conventional monolayer cultures of the same cells.
Similar(13)
But this is tricky to achieve when a single, anodyne culture – the culture of the same global market – is flooding the ground everywhere.
Nearby is a larger mantle, also from the Paracas culture of the same era, but in a much better state of preservation.
Carefully assembled from private collections, the exhibition made a firm statement against the conservatism of public gallery culture of the same period.
We hypothesized that if cytokine consumption by Treg in the vicinity is responsible for apoptosis in Teff, culture of the same number of Teff and Treg in a smaller volume should enhance suppression mediated by apoptosis induction.
A parallel culture of the same cells was grown at 3% O2 to prevent sense-cence.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com