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10 ml YM overnight cultures from a single colony of Rouxiella sp.
To determine their mutation rates, six independent cultures from a single original colony were established for each of the 21 strains.
The alkaline phosphatase activity of treatment cultures was expressed as a percentage of control cultures (n = 6; 6 independant cultures from a single donor cell source).
Out of the 8 cases in the aseptic loosening group with positive conventional cultures, 3 cases had positive cultures from a single sampling technique.
To investigate potential quantitative and qualitative changes in HERV-W expression following influenza A virus infection in primary human cells, fibroblast cultures from a single individual were studied.
To determine the dose dependence of the radiation-induced effect, replicate cultures from a single specimen (B1450) were exposed to X-ray doses of 0, 5, 25, 50, 100 and 200 cGy and progeny cultures were analyzed for SA-βgal activity four to six weeks following irradiation.
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We defined ABU as the isolation of urinary GBS in pure culture from a single sample, which is an approach consistent with prior studies [ 14, 41].
An overnight culture from a single colony was added to 2 × YT broth supplemented with 0.2% glucose and 100 μg/ml ampicillin.
Cells expressing that phenotype had high cloning efficiency in culture from a single cell, generating mixed colonies containing luminal cells and myoepithelial cells.
As seen in BCG sub-strains, variation in colony morphology among isolates of M. tuberculosis from patients is extremely common and multiple phenotypes are often apparent even from a microbial culture from a single sputum sample.
Overnight starter cultures grown from a single S. cerevisiae colony were used to inoculate large YPD cultures to a final optical density (OD660) of 0.05.
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