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Compared to mammalian cultures, expression in P. pastoris is cheaper and faster, potentially leading to decreased costs and process development times.
There are several other notable features such as self-renewal, the capacity to form tumor spheres in low-adherence cultures, expression of high levels of ATP-binding cassette (ABC) drug transporters (especially ABCG2) and multi-drug resistance.
In ICI-treated cultures, expression of PyLT increased the percentage of ICI treated cells in S phase between 12 and 24 h, from 6 7 percent at the 0 h time point to 20 percent by 24 h (Figure 3A, upper panel).
In neurosphere cell cultures, expression of Id3 was also dependent on FGF2 (Nieto-Estevez et al. 2013).
In these same cultures, expression of recombinant Sema5A in pyramidal neurons leads to a reduction in spine density.
While the expression data for the exponential growth phase was derived from triplicate cultures, expression data for the three post-exponential time points was obtained from duplicate cultures.
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"Out" was just a reaction to the youth culture expression "in" (as in, with the in-crowd).
After culture, expression of the gap-junction protein connexin 43 was clearly observed intercellularly in isolated clusters.
Here we report on recent advances in cell culture expression and functional characterization of human rADAMTS-13.
Target regions are tested for accessibility in a human cell culture expression system where the hhRz RNA and target mRNA and protein are coexpressed.
To borrow a pop culture expression, it was clearly an epic fail for thousands of them.
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