Sentence examples for cultures establishment from inspiring English sources

EM contributed to the study concept and design, was responsible for cell cultures establishment and handling and contributed to writing of the manuscript.

C.M. and T.B. conducted pre-experiments regarding the co-culture establishment.

See Gribble and Anderson [18], [22] for details of the protocol used for strain isolation and culture establishment.

For tobacco cell culture establishment, N. benthamiana leaves were cocultured two days with A. tumefaciens in the dark at 26°C, rinsed in a liquid MS medium containing 3% sucrose and 150 mg/l cefotaxime (Sigma).

Immature inflorescences are a significant resource for in vitro culture establishment.

The detailed method of primary culture establishment can be found elsewhere (Kang et al, 2000).

SM carried out cell culture, establishment of cell systems and molecular genetic studies and participated in preparation of the manuscript.

The seasonal effect on culture establishment has also been reported in Azadirachta indica (Arora et al. 2010) and Salvadora persica (Phulwaria et al. 2011).

The DNA-damaged cells co-expressed the replication markers TPX2/repp86 and centromere protein F. Once damaged in the early stages of culture establishment, fibroblasts continued to express DNA breaks with each replicative cycle.

At the time of culture establishment, we arbitrarily selected ∼8 30 individuals to be genotyped with 99 amplified fragment length polymorphism markers (AFLP markers) using the methods of Zuellig and Thum (2012).

However, the generation of EBV-CTLs or EBV peptide-specific CTLs from peripheral blood is a complex, time-consuming, and laborious process, which includes setting up EBV-positive transformed B lymphoblast cell lines (LCLs), limited dilution cloning, culture establishment by in vitro restimulations, and genetic engineering.