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Cultures conditions for cytotoxic activity screening assays.
This includes progress and understanding in biomaterials science (cell culture substrate), biomechanics (dynamic cultures conditions) and biology (tissue engineering).
In this work RANKL was cloned for expression at high levels in Escherichia coli with the interaction of changing cultures conditions in order to produce the protein in a soluble form.
Thus, DNA damage accrual is not a response to artificial cell cultures conditions (as previously discussed in aging fibroblasts), but may be part of both intrinsic and extrinsic mechanisms governing the physiological aging process in human stem cells.
CAZyme distributions were compared according to the different cultures conditions.
N. oleoabundans was able to grow under strict heterotrophic cultures conditions with glucose or cellobiose as the only carbon source; however, no growth was obtained using xylose, arabinose, fructose, sucrose, lactose, glycerol or acetic acid.
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The diverse culture conditions viz.
Figure 2 Influence of cell culture conditions.
The culture conditions were 37 °C, 5% CO2.
Subconfluent and confluent culture conditions were used.
Fig. 4 Effects of culture conditions on pyruvate production.
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