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Additionally, some bags holding SP700 resin were also deployed in a mixture of dinoflagellate cultures composed of A. tamarense and Prorocentrum lima, the latter being a diarrheic shellfish toxins (DSP) producer.
Only cell cultures composed of at least 99% epithelial cells were retained for RNA extraction.
Thus, cultures composed of mixed populations with distinct HPT alleles, such as those that arise during laboratory passage of B. pertussis, are readily identifiable by PCR/LDR.
To manipulate the microvascular niche, the authors generated organotypic cultures composed of mixed human umbilical vein endothelial cells (transduced with the adenoviral gene E4ORF1, which eliminated the need for serum and cytokines) and either lung fibroblasts or bone marrow-derived mesenchymal cells.
The IC50 values reported in Table 1 show a very low susceptibility of primary cell cultures, composed of resting cells and macrophages from buffy coats to the effects of fullerenes 1 and 2. These cells showed no toxicity up to 72 h exposure to the maximum dose tested, and it was not possible to precisely extrapolate the IC50 in these cell lines.
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But they also point toward an increasingly atomized audience, a popular culture composed of a zillion nonintersecting mini-cults.
Weaving together fragmentary images of cartoon characters, professional athletes and body parts, they suggest a culture composed of a whole lot of everything in which nothing is whole.
These indices, measured using collagen-bonded scaffolds, were also higher than scaffolds coupled to fibronectin or an ECM control sandwich culture composed of type I collagen and Matrigel.
Artificial co-cultures composed of different Clostridium species were shown to offer better performance in terms of efficient H2 production than the corresponding pure strain cultures.
Therefore, H2 production using artificial co-cultures, composed of well characterized strains, is one of the directions currently undertaken in the field of biohydrogen research.
Four pure Clostridium cultures, including C. butyricum CWBI1009, C. pasteurianum DSM525, C. beijerinckii DSM1820 and C. felsineum DSM749, and three different co-cultures composed of (1) C. pasteurianum and C. felsineum, (2) C. butyricum and C. felsineum, (3) C. butyricum and C. pasteurianum, were grown in 20 L batch bioreactors.
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