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Overnight cultured type II cells on glass coverslips were stimulated with a combination of secretagogues (100 µM ATP, 0.1 µM PMA and 10 µM terbutaline) for 2 hrs.
For determining the effects of lung surfactant secretagogues on the lamellar body pH, overnight cultured type II cells were stimulated with lung surfactant secretagogues for various times.
To determine whether protein kinase C (PKC) and Ca2+/Calmodulin-dependent Kinase II (CaMKII) were involved in the Baf A1-mediated secretion, overnight cultured type II cells were treated with 20 nM Baf A1 and 100 nM staurosporine or 10 µM KN-62 for 1 hr and the zero time value was measured.
We demonstrated that exogenous surfactant alters DNA synthesis and viability of cultured type II cells differentially.
Consistent with the results observed in A549 cells, sorafenib could also blunt the TGF- β1-dependent reporter activity in primary cultured type II AECs.
Meanwhile, co-staining for ZO-1 and fibronectin revealed that sorafenib reversed the TGF- β1-induced EMT in primary cultured type II AECs.
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It ought to be read as a companion piece to The Rector's Daughter and Worth (below), all three speaking volumes about the joys and pains that await cultured types who attempt a permanent life in the East Anglian countryside.
Similar experiments were performed in cultured type-2 astrocytes isolated from neonatal rat brain.
Not that you cultured types would cheat.
No substantial difference was found between the expression levels of the two genes studied in cells cultured onto type IV and type V collagen substrates.
In some case, cells were cultured on type I collagen-coated microplates (CYTOOchips™Mini, CYTOO Inc, Cambridge, MA, USA) with adhesive micropatterns of different sizes and shapes.
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