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After that, inoculum sizes 5%% was transferred to submerged or solid cultured substrate.
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In this study, the role of substrate nanotopography as a major determinant of hepatocellular cytotoxic responses over a short time of exposure was reported on which nanopatterns with various geometries were utilized as cultured substrates for the hepatic cell line (HepG2).
The OSTs were cultured on substrates (3 × 104 cells/each substrate) and incubated for 4, 8, 10 days.
This difference is more pronounced when cells are cultured on substrates other than tissue culture polystyrene.
Herein, clones are seeded such that one clone per well is cultured, the substrates added, and the activity or products screened, primarily using chromogenic or fluorogenic substrates.
Fibroblasts cultured on substrates of variable stiffness, mimicking soft tissue environments, automatically increase their own rigidity to match substrate stiffnesses ranging from 500 to 40,000 Pa [ 472].
In addition, it was observed that the MTT activity was higher in cells cultured on substrates containing HA.
Cells are subjected to static tension of different magnitudes when cultured on substrates with different stiffnesses.
Cells cultured on substrates with smaller pitches exhibited a substantially higher frequency of cell alignment and smaller circularity index.
When cultured on substrates with topographical features of less than 10 nm neither cells nor their extracellular matrix showed organizational asymmetry.
Bovine aortic endothelial cells were cultured on substrates with microstructures between 2 and 5 μm in wavelength and with constant feature depth of 0.45 μm.
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