It's the world of white, cultured, self-confident, postwar America; east coast mostly, with a dash of the south.
ESCs cultured under self- renewing conditions proliferate quickly, yet appear to meet the majority of their energy requirements by glycolysis similar to classic Warburg cancer cells [22].
Sorted cells were then cultured in self-renewal conditions in the presence of EGF and FGF2.
Similarly, transgenic FLAG MKRN1 was also primarily visualized in the cytoplasm of cells found in ESC populations cultured in self-renewal or RA-induced differentiation conditions (Fig EV1E).
The neurospheres were then plated adherently on 100 mm dishes coated with poly-d-lysine and laminin, and cultured in self-renewal medium (see above for composition) for additional 2 days.
CNS neurospheres were formed by non-adherently culturing E13.5 wild-type dorsal telencephalon cells then plated adherently on 100 mm dishes coated with poly-d-lysine and laminin, and cultured in self-renewal medium for an additional 2 days.
Our results indicated that similar to hESCs, hIPS cells with depleted CENP-A exhibit no change in SSEA4 or apoptosis relative to control cells cultured under self-renewing conditions (Fig. 4E and F).
We next explored the dynamics of MKRN1 protein expression in undifferentiated and committed ESC populations by comparing MKRN1 abundance in whole-cell lysates obtained from bulk ESCs cultured in self-renewal (+LIF), or RA-induced differentiation (+RA) conditions by Western blot analysis.
That way, we can finally all pat ourselves on our backs in a haze of pseudo-cultured self-satisfaction, and slam everyone else for being complete fucking idiots.
Cells were cultured in monolayer, self-assembled pellets or collagen hydrogels.
