Sentence examples for cultured reference from inspiring English sources
The abundance of the organism's elliptical spores was greater in the patient's sputum culture than in cultured reference colonies [ 1].
Total RNA of sphere cultures and 2D cultured reference cells was isolated with the RNAqueous kit (Ambion Applied Biosystems) and cDNA synthesized with Superscript II reverse transcriptase (Invitrogen).
In vitro analysis of the cultured reference strains with the automatic sequencer as well as with agarose gel, showed banding patterns identical to the predicted in silico banding patterns.
73 74 Thus, they rely heavily on the availability of genome sequences and functional information from cultured reference bacteria, which means there are considerable benefits from combining different approaches to gut microbiota analysis.
DNA extraction Chromosomal DNA from cultured reference bacteria (Additional file 1: Table S1) was isolated using the Easy-DNA™ Kit (Invitrogen, Carlsbad, CA, USA), for further details on the DNA extraction protocol see Additional file 2. DNA was precipitated with ethanol, and resuspended in 60 μl TE buffer.
The trial, in Federal District Court in Manhattan, was a twisted, often dizzying affair with cultured references to identity, aesthetics, Melville, sexual abuse and the commercial side of art.
Although most of the LCs could be assigned to the class Spirotrichea, the low sequence similarities to the closest cultured references indicate that the taxonomic assignment should be taken with care.
All primers were tested against 15 strains of pure-cultured reference bacteria, representing targets for one or more of the different primer sets (Table 1).
In conclusion, the sequencing data confirmed the specificity of the primer sets found in the verification process of the Gut Microbiotassay when tested against DNA extracted from pure-cultured reference bacteria.
Known pathogens, including cell-cultured viral reference strains, cultured bacteria, and fungi, were used to verify EOPM performance.
The mini-exon PCR-RFLP (Restriction Fragment Length Polymorphism) genotyping scheme was validated with cultured WHO reference strains of Leishmania and cultured isolates from patients [ 51, 52].
