If you thought that Raymond Carver's men would be happier if only they had a little help — if they'd been educated, cultured, programmed, employed in white-collar rather than blue-collar jobs, sent to talk therapy and prescribed antidepressants — well, Robert Stone is here to tell you that none of that guarantees anything.
"We're cultured [and] programmed to just feel better when we have some sort of badge of distinction," he wrote, "and all the psycho-social and cultural goodies that are attached to said badge".
Unlike embryoid bodies, USSC spheres do not commit to any differentiation programs when cultured in USSC growth mediumACF.
Second, Streptomyces spp. undergo a developmental program when cultured on solid medium consisting of a substrate mycelial phase followed by the formation of aerial mycelia that septate to form spores.
A study focusing on the gene expression program in cultured primary fibroblasts in response to serum, believed to reflect the functional role of fibroblasts in wound healing, found that the "wound-response signature" was coordinately regulated in many human tumors and was a powerful predictor of the clinical course in several carcinomas [28], [29].
Moreover, ectopic expression of NT-PGC-1 α-a activates the muscle transcription program in cultured myotube cells, enhancing mitochondrial oxidative capacity.
Human Umbilical Vein Endothelial Cells (HUVECs) pooled from three different donors were obtained from the Yale Vascular Biology and Therapeutics program and cultured in M199 medium supplemented with 20% Fetal Bovine Serum, 50 µg.ml−1 of Endothelial Cell growth Supplement (ECGS) prepared from bovine hypothalamus, 100 μg.ml−1 heparin, 100 U.ml−1 penicillin and 100 μg.ml−1 streptomycin.
Our data demonstrate the existence of large-scale AR-dependent gene expression programs in fibroblasts cultured from human external genitalia.
This inefficiency may involve irreversible changes in the epigenetic program of the cultured cells.
This program envisioned returning cultured lands into lake areas and included removing embankments and relocating residents from schistosomiasis-endemic areas to newly established towns.
