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Thus, aggregation of DCs in the cultured network is associated with activity-enhanced synaptic clustering.
Figure 1 shows that MEAs can be used to investigate the dAPs of many neurons in a cultured network.
Hence, the presence of DCCs increases the number of neurons innervated by single axons in the cultured network.
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We used network engineering techniques to induce self-organization of cultured networks into neuronal clusters of different sizes.
Learning, or more generally, plasticity may be studied using cultured networks of rat cortical neurons on multi electrode arrays.
One possible explanation for the highly varying success of the training protocol is the balance that cultured networks may develop between activity and connectivity.
To perform electrical recordings from cultured networks using CNT-MEA chips, clean silicon chips were bonded to printed circuit board (PCB) supports and were adjusted with quartz tubes to contain the biological medium.
In addition, the ESWN topological property of the cultured networks was proven to be activity-independent, as pharmacological inhibition of synaptic activity did not affect the measured values, relative to control conditions (not shown).
At early developmental stages there is also very little internal activity in cultured networks (see Fig. 2g).
Baseline activity of the neuronal networks was measured in NDM prior to artificial or human CSF exposures which verified that MEA cultured networks showed initiation of functional activity detected as single spikes.
Our implementations of the optoclamp are quite reliable for controlling neural activity in cultured networks and thalamic cells in vivo and the technique's simplicity lowers the barrier for its adoption.
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