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Source tissue, bacterial identities based on 16S rDNA homology, TRFLP fragment sizes, and in vitro functional traits of cultured maize root and shoot endophytes.
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Funneliformis mosseae (F.m was cultured under maize with 90% of root colonisation, and Rhizophagus intraradices(R.i was cultured under maize with 84% of root colonization.
The fungus was cultured using maize cobs as a carbon source.
To test the hypothesis that gene expression by the fungal partner in this beneficial interaction is modulated by the plant, Trichoderma virens was co-cultured with maize or tomato in a hydroponic system allowing interaction with the roots.
The topoisomerase I inhibitor camptothecin (CPT) produces a reduction of maize cultured cell growth when added to the culture medium.
Maize cultured cells were incubated in the presence of different concentrations of camptothecin.
There was no maize cultured in neighbouring fields that had been sown at the same period of time, so that the probability of cross-pollination was minimised.
The aim of this study was to identify possible nucleases associated with the camptothecin inhibition of cell growth in maize cultured cells.
We have identified and characterized CaMNUC32, a 32kDaCa2+/Mg2+-dependentCa2+/Mg2+-dependentS1/P1 family induced by the topoiS1/P1ase I inhibitor camptothecin in maize cultured cells.
In this paper, we identify and characterize a Ca2+/Mg2+-dependent nuclease whose activity is induced by CPT in maize cultured cells.
The average size of the genomic DNA fragments extracted from CPT-treated maize cultured cells are significantly shorter than the extracted from untreated cells.
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