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Mr. Zhang's charge and curious disappearance add to a growing impression that Mr. Bo's aggressively cultured image of Chongqing as a model for China's future — moral and crime-free, rapidly urbanizing, growing like a weed — has been tarnished.
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DMZ explants for the analysis of convergent extension movements and time-lapse experiments were prepared at stage 10.25 and cultured, imaged and scored as described previously (Unterseher et al., 2004).
Blastocysts were collected from E3.5 embryos, cultured and imaged in vitro for 8 days followed by genotyping.
Files were cultured and imaged at 24°C.
Slices were prepared, cultured and imaged as previously described [ 9, 30] (supplementary Methods online).
The training set was cultured and imaged 18 months prior to the test set.
To determine whether LAR-GECO1.2 could be used to image these activity-dependent mitochondrial Ca2+ elevations in neurons, we imaged cultured peripheral and central neurons using a mitochondrially targeted form of the indicator, mito-LAR-GECO1.2.
Cells were cultured, stained and imaged in 6-well glass bottom dishes (In Vitro Scientific).
The next day, ~30 worms per strain were imaged, and the remainder cultured on OP50 and thereafter imaged consecutively for 6 days.
Measurements were carried out at 25°C. Figure 3b is an SEM image of GH3 cultured in the same location as that shown in Figure 3a by seeding the cells of passage 10.
The cell colonies cultured in 3D were imaged and used for immunofluorescence staining at Day 4 after seeding.
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CEO of Professional Science Editing for Scientists @ prosciediting.com