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The cells were cultured further and the generation time in each culture system was counted three times.
The latter was isolated as a single colony from a 90°C culture of an autoclaved mud sample and could not be cultured further.
After transfection, the cells were cultured further for additional 2 3 days before inspection using a laser scanning confocal fluorescence microscope (FV1000, Olympus), and the transfected cells were identified according to the fluorescence signals of Dendra2 and TagRFP.
UV visualised embryos were not cultured further.
Cells that could proliferate in the absence of OP9 cells were cultured further.
Then, neurospheres derived from the cells were layered and cultured further on PDL-laminin double-coated well plates.
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If no CPE developed in the inoculated cultures, the cells were cultured for further 7 days.
Thereon, culture medium was replaced by the experimental culture mediums and cells were cultured for further 24 hours.
The cells were usually cultured for further 18 h, and the cells transfected with the tRNase ZL siRNA or the Ago2 siRNAs were cultured for further 42 h.
A total of 1084 mutants were separated and cultured to further analyze their adhesive and invasive capabilities.
Tumor cells were continuously cultured for further investigation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com