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We also attempted to culture other bacteria and fungi species, but not all were successfully cultured (data not shown) since some species were slow-growing or difficult to culture, and the samples were stored at −80 °C before use.
In addition, 28 and 16 Synechococcus host and phage sequences were extracted from cultured data [See Additional file 1].
The number of bacterial colonies decreased from 2.3 × 10 CFU/mL to 10 CFU/mL over 120 days, and they could still be cultured (data not shown).
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‡Patients with specimens cultured, MIC data available and presented for all 4 isolates.
In addition, we observed that wound healing of Human microvascular endothelial cells (HMEC) treated with supernatants from CD4+ or CD8+ TIS-T cell/Mo-Ma CCs exhibited a similar wound closure than HMEC treated with supernatants of control-T cells/Mo-Ma CCs or Mo/Ma cultured alone (data not shown).
Given the large confidence intervals generated by BayesAss+ with the laboratory-cultured data sets, we generated in-silico simulated data sets both under conditions similar to our in-vitro experimental design (see Methods) and with an increasing number of sampled individuals and/or number of loci.
ROCK inhibition is similarly effective for hESC cultured on MEF (data not shown).
TGF-β1 and IL-6 mRNA levels were unchanged upon challenge with supernatants from wild type and mutant bacteria cultured in BHI (data not shown).
In striking contrast to the in vivo microdialysis data, cultured astrocytes preloaded with [3H]taurine failed to show any increase in taurine release levels when perfused with the same low [NaCl] isoosmotic medium (Fig 2a).
We do not believe that this is the case because the decreased body size immediately reverted to normal size within one generation when AF flies were cultured in normoxia (data not shown), which is very different from the trait of hypoxia tolerance that was shown to be heritable in the AF flies.
Unc5B effect on adhesion was specific and not due to cell toxicity or apoptosis, since (i) the detached cells remained viable and proliferative when cultured ex vivo (data not shown), (ii) deletion of the pro-apoptotic Death domain did not abolish cell deadhesion (Figure 2A, Unc5BΔD) and (iii) the phenotype could be rescued by C-cadherin overexpression or by Rnd1 depletion (see below).
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