BTE has been reported to lack cytotoxic effects on cultured cells, consistent with our findings [ 10].
This observation suggested that high ABA concentrations (such as 50 or 100 μM) in MS media cause a negative effect on cultured seed development, consistent with previous observations in rapeseed cultures and Lesquerella fendleri cultures [ 7, 24].
The results obtained in this study using the phenotypic method of screening of cultured bacteria are consistent with what has been reported in the literature.
After 3 d of exposure to 5μMM of gossypol, the parent A549 cells and control A549 cells with an integrated RFP hairpin showed 81% and 77% survival, respectively, compared with cells cultured without drug, consistent with our initial dosing experiments.
We conclude that both Cap-H2 and Mrg15 are required to maintain interphase chromosome compaction in cultured Kc cells, consistent with a condensin II axial shortening function (Shintomi and Hirano 2011; Bauer et al. 2012).
Our results both in vivo and in vitro in cultured cardiomyocytes are consistent with a deleterious effect of imatinib on the heart that is dose, time, and age dependent.
We conclude from these initial experiments that miR-34a over-expression has a pronounced anti-proliferative effect on NB1691luc and SK-N-ASluc cell lines cultured in vitro, consistent with prior publications [ 6, 8].
The interaction between HA- CD44 is critical for modification of this communication axis during CCs expansion [ 101] and relatively higher expression of these molecules in cultured CCs is consistent with the notion that HA- CD44 interactive effect is vital for oocyte maturation [ 100].
These same investigators took monocytes from human volunteers and cultured them; the cultured cells expressed markers consistent with fibrocytes (CD34 + CD45 + Col I + α-SMA +) and were morphometrically similar to the vascular smooth muscle cells found in intimal hyperplasia [ 45], suggesting an association between intimal hyperplasia and fibrocyte migration.
Our results with IL-2-cultured TIL are consistent with the hypothesis of an IL-2-induced modulation of the markers involved in activation mechanisms, manifesting as a restoration of the ɛ and ζ chains and, to a lesser degree, of p56lck expression, an increase in FasL expression and an elevated perforin production.
