In addition, seven coded cultured isolates were sent directly from Alabama to a laboratory in Korea (Ahn JH) for independent DNA extraction, PCR and sequencing.
Briefly, the total mRNA was extracted from cultured cells and genes coding for the light chain and Fd fragment were amplified by RT-PCR, as previously described (CSH press, Phage display manual, ed. D.R.Burton, pag. A1.10).
"Above all," says Willem, "it means to be funny, and cultured enough to understand the code of humour.
"This is cultured cow milk".
From the MTT assay we expected that the cells cultured in Matrigel express genes coded for the proteins required for ECM interaction in Matrigel, indeed the real time PCR assay showed that among the 84 genes analyzed, 30 were detected in the Matrigel scaffold, but only few in the designer peptide scaffolds.
(B ) Pseudo-color coded image showing cultured neurons expressing GCaMP6f.
To allow rapid adaptation of the technique we optimized an application protocol of the CSMA cell patterning method for 85 cell types and applied the platform for functional genetics profiling of G-protein coupled receptor coding genes in cultured prostate cancer cells and non-malignant epithelial prostate cells, demonstrating the potential of the CSMAs for context specific target discovery.
Cultured Purkinje cells recapitulate an intrinsically coded neuritogenic program, involving initial navigation of the axon towards the target field and subsequent expansion of the terminal arborisation.
To identify hAMSCs in our in vivo mouse experiments, we transduced primary cultured P1 cells with lentiviral vectors coding for GFP/luciferase proteins.
Lastly, H3.3 is accumulated on transcription factor binding sites in the regulatory regions of most genes as well as on the coding regions of transcribed genes in cultured mouse ES cells [ 6], and in cultured human HeLa cells [ 17].
