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The remaining cells were cultured, changing the medium every 2 d.
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The non-adherent cells in the culture were removed by washing the culture dishes with PBS, and the adherent cells were cultured by changing the culture medium every 3 days until the cells became nearly confluent after 10 15 days of culture.
The adherent cells were cultured by changing medium every 3 days and the cells became nearly confluent after 14 days culture.
Four mL were added per well of a 6 well plate and the cells were cultured for 7 days without changing the medium.
The data suggest that these transcription factors are activated by changing the cultured environment from 2D to 3D.
We also examined induction of p27Kip1 in cultured monolayer keratinocytes after changing in the medium to one containing a high [Ca2+].
In addition, we examined DNA synthesis in cultured monolayer keratinocytes after changing the medium to one containing a high Ca2+ concentration ([Ca2+]), which induces keratinocyte growth arrest and differentiation.
While at UCLA, she also developed interests in the capacity of corals to acclimatize to changing environmental conditions, and cultured diverse skills in molecular genetics, which together laid the groundwork for her engagement in the concept of assisted evolution.
NP cells were cultured in 500 μl culture medium (37 °C, 5%% CO2) by changing the culture medium every two days.
When the piano keys are played, the cultured slime mould responds by changing its shape, and this movement creates electrical energy.
Furthermore, we have previously shown that changing perfusion of cultured BCEC between bicarbonate-free to bicarbonate-rich conditions has no effect on [Ca2+]i [ 28].
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