Sentence examples for cultured atmosphere from inspiring English sources

You will feel instantly at ease in this cultured atmosphere.

Burnshaw grew up in a cultured atmosphere among 19th-century literature and lieder, which continued when the orphanage moved to Westchester county.

In the cultured atmosphere at home in the Bronx, Doctorow read widely, "everything from boys' adventure stories to sports novels to Dostoevsky.

The Adam children grew up in the cultured atmosphere of a propertied and well-connected 18th-century family.

The apartment had a friendly, cultured atmosphere, with a grand piano, a great many books, photographs of friends and family, abstract, modernist paintings on the wall, and mementos of their trips on every available surface.

Though he flourished in the cultured atmosphere of the analyst's home, the treatment did little to banish the allure that suicide held for him, which manifested itself repeatedly in his work.

Next, the cell suspension was transferred to a 25 cm culture flask and cultured in humidified atmosphere containing 5% CO2 at 37°C.

All cells were cultured in humidified atmosphere of 95% air and 5% CO2 at 37°C.

Cells were cultured in humidified atmosphere of 5%CO2/95%% air at 37 °C and were sub-cultured using 0.025% trypsin/0.01% EDTA.

Stably transfected HEK 293 cells were adapted to suspension culture in a spinner flask using 293 SFM II medium (Invitrogen, Gibco), and cultured in an atmosphere of 5% CO2 in air at 37°C for three days.

After centrifugation (1,400 g, 20°C, 20 min) the cells were aspirated from above the interface, washed once in serum-free MEM and subsequently cultured under an atmosphere of 5% CO2, 95% air in 100-mm tissue culture dishes using M199 containing penicillin G 100 U/ml, streptomycin sulfate 100 U/ml and 10% FBS.