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(d) Cell culture temperature.
(h) Cell culture temperature.
Moreover, GW9508 significantly induced the glucose oxidation and tended to increase the cell culture temperature (Supplementary Fig. 6).
The culture temperature is then increased to 37°C, collapsing the hydrated grafted chemistry.
HepG2 cells growing on the modified surfaces could be recovered spontaneously by only reducing culture temperature.
In addition, the optimum culture temperature and pH were 30 °C and pH 7.0, respectively.
The attached cells could be recovered simply by lowering culture temperature.
The optimized condition shall be initial pH 5.7, moisture content 72% and culture temperature 30°C.
The culture temperature was then lowered to 20 °C and protein production induced by the addition of 100 µM isopropyl β-d-1-thiogalactopyranoside.
(b) A decrease in culture temperature: for the dark-grey response we used a PI controller during the 37 °C phase to achieve the desired sfGFP fluorescence.
Total Se yield was significantly affected by culture temperature (P < 0.05), initial pH value (P < 0.01) and volume (P < 0.01).
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