Sentence examples for culture sequences from inspiring English sources

For the remainder of the putative fungal isolates identified, whether the tissue culture sequences represent fungal microorganisms that are the same species or genus as their closest GenBank match is unclear.

The 16S rRNA gene from the pure culture sequences from the NCBI database were aligned using Clustal X (Thompson et al. 1997) and the maximum likelihood (ML -based phylogenetic tree was constructed using ML -basedsion 6) (Tamura et al. 2013).

The majority of the GenBank taxa with close sequence identity matches to the tissue culture sequences were fungi known to have conifer associations, had been isolated as fungal endophytes from other plant species or had been obtained from environmental samples from forest settings, according to their GenBank accession details.

Furthermore, datasets were dereplicated (when identical, only unique sequences were kept for each sampling site in the BioMarKs database), which resulted in a final dataset of 25,842 unique BioMarKs sequences (8,931 DNA and 16,911 cDNA sequences, respectively), 308 ciliate culture sequences and 928 ciliate sequences of earlier environmental studies (listed in Additional file 1: Table S1).

Placing the Still Bay within the MSA culture sequence has been problematic in the past because Still Bay assemblages are rarely found in situ and previous excavations were inadequately recorded.

As proof of principle that this approach works, we tested if MECs in which the β1-integrin gene had been excised in 3D culture, showed integrin protein loss and cell cycle defects after replating the cells onto 2D substrata (i.e. using the culture sequence similar to that shown in Fig. 4ii).

Strain isolation, culture, sequencing, and variant calling are detailed in the original publications [ 15, 16].

In all environmental datasets, only sequences which could be assigned to a culture sequence belonging to the phylum Ciliophora and had at least 300 bp in length were considered, amounting to 85,482 BioMarKs ciliate V4 SSU-rDNA sequences.

The sequence from the ECT.2 sample acetic-acid-producing culture (sequence ECT.2c1) has 100 % BLAST identity to Acetobacter lovaniensis (accession # NR_114845, Cleenwerck et al. 2002), and is related to OTU 1 (100 % identity), which accounts for ~53%% of the pyrosequencing library from sample ECT.2.

The sequence from the ECT.1 sample acetic-acid-producing culture (sequence ECT.1c1) has >99 % identity to Acetobacter aceti (accession # NR_113549, Iino et al. 2012) and is closely related to OTU 4 (>99 % identity), which comprises ~18%% of the pyrosequencing library generated from sample ECT.1.

In the first part of this study a strategy of three-culture sequences was developed to characterize hydrogen production by four pure Clostridium strains (C.