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After three rounds of panning, 180 μL of ER2738 cell culture (mid log phase, OD600 = 0.5 AU) was resuspended with 10 μL of diluted phage eluates.
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Specifically, following 1 week of culture, mid-term markers of various MSC lineages were examined in order to assess the strength and specificity of the observed osteogenic responses.
Dawenkou culture (mid-5th to at least mid-3rd millennium) is characterized by the emergence of wheel-made pots of various colours, some of them remarkably thin and delicate; vessels with ring feet and tall legs (such as tripods, serving stands, and goblets); carved, perforated, and polished tools; and ornaments in stone, jade, and bone.
For primary cell culture, mid-pregnant animals were sacrificed and abdominal mammary glands excised and washed in 70% ethanol.
To understand if C5a contributed to the activation of primary HSC during culture, mid-quiescent primary HSC (day 5) were treated with or without 10 ng/mL C5a until day 8.
This phenomenon is illustrated for yeast cultures (mid log-phase) that are quickly heated within seconds using a thermocycler.
A culture of mid log-phase yeast cells (OD600 ~ 0.5) was divided into four equal samples.
After addition of MMS, cells were returned to 30°C incubation with light shaking for 60 min. A second culture of mid log phase yeast cells was divided into four equal samples.
This was his Ghana, a crossroads culture, the mid-twentieth-century version of globalization (as always, a function of empire).
Record companies have discovered they can sell club culture to mid-30s couples who would never dream of setting foot in the Ministry of Sound.
It says a lot about the class-ridden culture of mid-50s Britain and paints a still-resonant picture of a younger generation educated but with nowhere to go.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com