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The cells were cultured under hypoxic conditions (<1% pO2 and 5% pCO2) using an anaerobic culture kit (Mitsubishi Gas Chemicals, Tokyo, Japan).
To some students of popular culture, Kit symbolizes a way of life that, harsh as it was, seems in retrospect to have been more stable, emotionally rich and rewarding than the lives we know.
First, in GS cell culture, Kit+ cells proliferated as actively as Kit− cells and frequency of SSCs was comparable between the two populations.
The collagen gel culture kit was purchased from Nitta Gelatin (Osaka, Japan).
Human chondrocyte conditioned media were screened using a human proinflammatory 7-plex MSD tissue culture kit (Meso Scale Discovery, Gaithersburg, Maryland).
Multiplexed cytokine analysis was performed to measure cytokine release from the PBMCs into the culture supernatants in response to stimulation using the MSD® 96-Well MULTI-SPOT® Human Cytokine Assays tissue culture kit (Meso Scale Discovery (MSD), Gaithersburg, MD, USA).
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California is not the only state to attempt to halt sales of the milk culture kits.
Individual IL-6 and IL-8 MSD tissue culture kits were used for further confirmation.
Human IL-6 and IL-8 were measured individually using MSD human cytokine assay tissue culture kits (Meso Scale Discovery).
In addition, approximately 0.1 g from each faecal sample was inoculated into an InPouch™ TF-Feline culturing kit (Biomed Diagnostics, USA) with a small plastic loop.
By PCR, 11 of 52 samples (21.2%) from 10 different households (21.3%) tested positive for T. foetus-DNA, including the sample that was positive for T. foetus using the culturing kit.
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