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The culture composition of the semi-continuous culture showed no weed algae hours after the inoculation.
However, previous studies often lack detailed isolation protocols or a precise characterisation of the culture composition excluding especially contaminating fibroblasts.
The main conclusion of this study was that the culture composition affected the biosurfactant production by C. ingens.
Both techniques were specifically adapted for use on native state bioleaching samples, with a view to establishing convenient means for monitoring culture composition.
Culture composition was determined microscopically (using a Zeiss Axiostar Plus light microscope, Carl Zeiss GmbH, Germany and a Thoma Neu chamber with a depth of 0.1 mm Paul Marienfeld GmbH & Co. KG, Germany).
The photosynthetic yield (Y) was proven to be influenced by numerous different factors like nutrients (Lippemeier et al. 2001), light, culture density (Schreiber and Klughammer 2013), and salt stress (Lu and Vonshak 2002) and culture composition (Kolbowski and Schreiber 1995).
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To monitor the co-culture composition, each strain was labeled with a fluorescent protein and counted by microscopy.
The culture morphogenetic composition during the process was monitored by a recently developed vision sensor.
We found that culture medium composition was more important than co-culture for expression and maintenance of albumin production.
To further enhance RL2 production, statistical experimental design methodology was applied to optimize the culture medium composition favoring RL2 synthesis.
Central composite design and response surface methodology were adopted to derive a statistical model for optimizing submerged culture medium composition.
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