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Plans have since been approved to add a $50 million philharmonic hall and a showcase for Hungarian folk traditions to the Millennial City Center's "culture block".
A piece of culture block (10 × 15 × 3 mm) obtained near the center of the colony was placed in 15 ml sterile distilled water in a 6-cm plate, and transferred to 4°C for 30 min to induce zoospore release from sporangia.
Single-conidium isolates were obtained by shaking a culture block (5 × 8 × 3 mm) of isolate KVF-2, grown on 10% V8 agar (10% V8 juice, 0.02% CaCO3 and 2% agar) (Ko et al.,2010a) at 24°C under light for 12 days, in 1 ml sterile distilled water in a test tube on a Vortex Mixer for 30 s to suspend conidia in water.
Briefly, BAC clones were transferred from 384-well plates to a 96-well culture block, which contained 1.5 ml of 2X YT medium with 12.5 μg/ml chloramphenicol and grown at 37°C overnight with shaking at 300 rpm.
White colonies from these two sets were carefully picked and used to inoculate single cells of a 96 well culture block with each well containing 1.2 ml of TB (1.2% Tryptone, 2.4% yeast extract, 0.4% glycerol) supplemented with 50 μg/ml carbenecillin.
Yeast cell cultures (OD600 ∼0.5) were centrifuged at 5000× g for 7 min, washed once in 1 ml PBS buffer (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mMKH2pH7.4pH7.4), transferred to a 96-well culture block in the case of 30 ml cultures, and centrifuged a second time at 5000× g for 7 min. The cell pellets were then frozen at −80°C.
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That culture blocks vital investment just to avoid sensible tax increases.
The British Museum had requested loans of 35 items for its current exhibition "Hajj: Journey to the Heart of Islam," but the ministry of culture blocked them, leaving the museum to find alternatives on short notice.
Culture is the pest always sneaking up from behind, clamping its clammy paws over the eyes and shrieking, "Guess who!" There is no shaking off Culture, blocking the path to any ledge in all the ranges of knowledge — the sciences and the social sciences, the humanities and the arts — from which we can look out and see.
Both the sequencing and original culture blocks were stored at -80°C.
Both the sequencing and the original culture blocks were stored at −80 °C.
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