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While this may have been due to high rates of cell death that correlates with loss of Venus expression in primary lymphocytes upon extended in vitro culture, application of IPTG in the drinking water also failed to drive transgene re-expression in peripheral blood (not shown).
In tissue culture application of a Ca2+-ionophore increased PGC-1 α levels.
In bacterial culture, application of lysin caused morphological changes of bacterial cells and ultimately led to cell lysis.
Everolimus (42- O- 2-hydroxy) ethyl-rapamycin) (RAD-001) was kindly prO- 2-hydroxy D Evans (NO- 2-hydroxymaceuticals, Basel, Switzerland), and dissolved in 100% DMSO for cell culture application.
A tetrazolium salt, 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) working solution was prepared just prior to culture application by mixing 1 ml of XTT stock solution (1 mg/ml in phosphate buffered saline (PBS)) with 10 μl of phenazine methosulphate (PMS) (1.53 mg/ml in PBS).
The council's head of culture, councillor Terry Geraghty, described the partnership as the "icing on the cake" of Hull's City of Culture application.
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The system includes three fluorescent channels and brightfield to simplify fluorescence imaging for cell culture applications.
The matrices were fabricated, biophysically characterized and optimized for cell culture applications.
This study presents a microfluidic PDMS platform for multiplex cell culture applications.
Calcium is widely used in all classic media, which is utilized in biomanufacturing and in the tissue culture applications.
The water-binding ability of the scaffold is an important function of biomaterials used for tissue culture applications.
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