Your English writing platform
Discover LudwigExact(3)
Most notably, cell culture adaptive mutations were identified in various non-structural proteins that enhance RNA replication by several orders of magnitude.
Western blot confirmed the presence of mature NS2 (JFH1 strain only due to genotype-specific antibody reactivity) while sequence analysis of SGR RNA extracted from the stable cell lines did not reveal any additional culture adaptive mutations.
As transient SGR for genotype 3a are not available, we utilized Huh7.5 cells stably harboring the genotype 3a derived SGR-Feo-S52 containing either the AII (T1056A, T1429I and S2204I) or SHI (P1220S, D1430H and S2204I) set of culture adaptive mutations (Saeed et al., 2012).
Similar(57)
The JFH-1 system has also been used as a backbone to construct inter-genotype chimeras, but these often show poor replication kinetics and acquire cell-culture adaptive mutations (Gottwein et al., 2009; Pietschmann et al., 2006).
There is emerging evidence that at least some culture-adaptive mutations render the strains more sensitive to antibody neutralization (Dhillon et al., 2010; Grove et al., 2008).
Not all culture is adaptive.
An effective culture is adaptive.
A previous study showed that influenza viruses often differ from those present in clinical specimens after isolation in MDCK cell culture as adaptive changes occur during virus transmission from the human host to cells of heterologous origin.
Admittedly I didn't have much time to consider the implications of adaptive culture in Berlin.
An adaptive culture is an antidote to groupthink.
An adaptive culture also encourages learning behaviors and discourages defensive ones.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com