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Cultural methods were used for bifidobacteria, Bacteroides, enterobacteria, enterococci, lactobacilli, and total anaerobes; culture-independent methods included denaturing gradient gel electrophoresis (DGGE) and quantitative real-time PCR (qRT-PCR) using Bifidobacterium-specific primers.
Additionally, 216 fecal samples from cattle were collected and tested by multiplex PCR and cultural methods.
We also cover the cultural methods that offer scope to support synergies between the aforementioned methodological combinations.
Cultural methods, the gold standard for bacterial detection, are hampered by alginate overproduction and the possible presence of dormant cells.
Cultural methods such as crop fertilization can affect susceptibility of plants to insect pests by altering plant tissue nutrient levels.
Molecular techniques have been proposed in recent years as a valuable alternative to traditional microbiological cultural methods, having numerous advantages (speed, specificity and sensibility).
Similar(28)
Escherichia coli and Staphylococcus aureus detected by cultural method in this study and elsewhere were not picked up by culture-independent method.
The cultural method revealed diverse but limited number of bacteria.
Solid-state fermentation (SSF) is a cultural method that holds tremendous potentials for the production of numerous microbial value-added compounds in various industries.
This "profil cultural" method was designed as a field diagnostic tool to identify the effects of tillage and compaction on soil structure dynamics.
Environmental DNA sequencing revealed a complex and flexible composition of the kombucha microbial culture (KMC) constituting more bacterial and fungal organisms in addition to those found by cultural method.
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