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In this review, various physiological, engineering parameters, and selection of proper cultivation strategy (fed-batch, two-stage etc).
Single-stage cultivation strategy is more favorable than two-stage cultivation owing to the lesser technical obstacles and being more cost-effective in the microalgal biodiesel production.
In this study, a biphasic cultivation strategy for a Chinese hamster ovary (CHO) cell line expressing an erythropoietin fusion protein (Epo-Fc) was developed.
This cultivation strategy was also efficient with other volatile fatty acids (butyric and propionic acids) or with a mixture of these three VFAs.
A widely applicable cultivation strategy, which reduces the costs of expensive isotopes, is designed for maximal (98 100%) incorporation of [13C] and [15N] into labelled recombinant protein expressed in Escherichia coli, allowing better assignment of the resonances for NMR studies.
Yields were improved by balancing expression levels of each gene, using a fed-batch cultivation strategy, and adding a solvent to the culture for extracting the product from cells.
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Novel cultivation strategies such as mixed substrate feeding are discussed.
The distinct capability of the mathematical model to predict highly dynamic fed-batch cultivation strategies was demonstrated by experimental implementation of two fed-batch cultivation strategies.
Various cultivation strategies employing different host strains and expression systems have been successfully employed for the production of recombinant proteins.
Resina, D., Cos, O., Ferrer, P. & Valero, F. Developing high cell density fed-batch cultivation strategies for heterologous protein production in Pichia pastoris using the nitrogen source-regulated FLD1 promoter.
Various cultivation strategies were designed for the production of poly 3-hydroxybutyrate-co-3-hydroxyvalerate) copoly 3-hydroxybutyrate-co-3-hydroxyvalerates poly 3-hydroxybutyrate-co-3-hydroxyvaleratels.
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