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Pig slurry rates of 0, 20, 40 and 80 m3 ha−1 were applied, scattered on the surface, before the sowing of each species in a cultivation sequence.
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To examine how the C. elegans Bristol wild isolate has evolved during laboratory cultivation we sequenced the LSJ1 strain of this isolate using Illumina technology.
KN performed the cultivation and sequencing experiments.
To overcome potential biases related to laboratory-reared populations, cell culture, or cultivation, we sequenced the genome of CRt cells that were purified directly from a natural tick population.
Here we report draft genomes of five species of the Sulfobacillus genus (AMDSBA1-5) reconstructed by cultivation-independent sequencing of biofilms sampled from the Richmond Mine (Iron Mountain, CA).
However, with the advent of cultivation-independent sequencing techniques, microbial communities of many environments have been characterized, including soil [ 5], the Arctic and Antarctic Oceans [ 6], and the Sargasso Sea [ 7].
Difficulty in RV-C cultivation and target sequence variation has precluded the development of a PCR based quantification method.
Moreover, STIRRUPS classification results can be used to identify samples containing novel organisms for cultivation efforts, genomic sequencing, and whole metagenome shotgun sequencing studies.
A further improvement in mixed-sugar utilization was obtained by prolonged anaerobic cultivation in automated sequencing-batch reactors on glucose xylose mixtures.
Beneficial soil nematodes are usually more abundant in crop management systems subjected to sophisticated crop sequences, cultivation practices and organic amendments.
Table 1 Cultivation techniques and sequences using summer catch crops.
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